|
KMID : 0380620100420040424
|
|
Korean Journal of Food Science and Technology
2010 Volume.42 No. 4 p.424 ~ p.430
|
|
|
Enhancement of Immune Activities of Peptides from Asterias amurensis Using a Nano-encapsulation Process
|
|
Jeong Hyang-Suk
Oh Sung-Ho
Kim Seoung-Seop
Jeong Myoung-Hoon
Choi Woon-Yong
Seo Yong-Chang
Choi Geun-Pyo
Kim Jin-Chul
Lee Hyeon-Yong
|
|
|
Abstract
|
|
|
|
Immuno-modulatory activities of peptides from Asterias amurensis were investigated using a nano-encapsulation process. The molecular weights of the peptides in the range of 5-7 kDa were separated using Sephadex G-75 gel filtration. Eighty-five percent of the nano-particles were in the 300 nm range using dynamic light scattering. The cytotoxicity of the A. amurensis nano-particles against CCD-986sk human dermal fibroblast cells was 11.64% after adding 1.0 mg/mL of the samples, which was lower than that from the control (13.28% collagen). The secretion of NO. from macrophages was estimated as 40 μM after adding 1.0 mg/mL of gelatin nano-particles, which was higher than the others. Prostaglandin E2 production from UV-induced human skin cells decreased greatly to 860 pg/mL after adding 1.0 mg/mL of the samples. Confocal microscopy revealed that nano-particles effectively penetrated the cells within 1 hour. From these results, we consider that nano-encapsulation of the peptides from A. amurensis can improve their biological functions.
|
|
|
KEYWORD
|
|
|
Asterias amurensis, collagen, cytotoxicity, nitric oxide, prostaglandin E2
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|